1IS9
Endoglucanase A from Clostridium thermocellum at atomic resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.801, 1.008, 1.006 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.441, 62.829, 103.891 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.030 |
| Rwork | 0.131 |
| R-free | 0.14900 |
| Structure solution method | MAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.030 |
| High resolution limit [Å] | 1.030 * | 1.020 |
| Rmerge | 0.049 * | 0.355 * |
| Total number of observations | 1605139 * | |
| Number of reflections | 158384 | |
| <I/σ(I)> | 14 | 3 |
| Completeness [%] | 98.2 * | 90.9 * |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 * | macroseeding, Souchon, H., (1996) Proteins: Struct.,Funct., Genet., 25, 134. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris | 0.1 (M) | pH7.5 |
| 2 | 1 | 1 | 0.2 (M) | ||
| 3 | 1 | 1 | PEG4000 | 17 (%) | |
| 4 | 1 | 2 | Tris-HCl | 0.1 (M) | pH7.2 |
| 5 | 1 | 2 | PEG8000 | 15 (%) |
