1IS4
LACTOSE-LIGANDED CONGERIN II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 61.400, 61.400, 80.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CONGERIN II- LACTOSE AND MES COMPLEX |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.970 |
| High resolution limit [Å] | 1.880 | 1.900 |
| Rmerge | 0.082 | 0.221 |
| Number of reflections | 12417 | 1159 * |
| <I/σ(I)> | 24.9 | 7.7 |
| Completeness [%] | 97.1 | 92.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 18 * | magnesium sulfate, sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 0.8 (M) | ||
| 2 | 1 | drop | lactose | 1 (mM) | |
| 3 | 1 | drop | protein | 1 (%(w/v)) | |
| 4 | 1 | drop | Tris-HCl | 0.1 (M) | pH6.5 |
| 5 | 1 | reservoir | 1.6 (M) | ||
| 6 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH6.5 |
