1IS2

Crystal Structure of Peroxisomal Acyl-CoA Oxidase-II from Rat Liver

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-08
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	72.030, 91.501, 214.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.209 *
Rwork	0.209
R-free	0.26000
Structure solution method	MIRAS
RMSD bond length	0.007
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.060	0.283
Total number of observations	344046 *
Number of reflections	69647	6152 *
<I/σ(I)>	16	2.1
Completeness [%]	94.7	85.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	PEG 20000, potassium phosphate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.0 (mg/ml)
2	1	drop	PEG20000	3.0 (%(w/v))
3	1	drop	potassium phosphate	20 (mM)	pH7.4
4	1	reservoir	PEG20000	8-11 (%(w/v))
5	1	reservoir	potassium phosphate	100 (mM)	pH7.4