1IRX

Crystal structure of class I lysyl-tRNA synthetase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-01-01
Detector	MARRESEARCH
Wavelength(s)	0.9792, 0.9795, 0.9824, 0.9724
Spacegroup name	P 1 21 1
Unit cell lengths	58.945, 74.767, 156.945
Unit cell angles	90.00, 90.26, 90.00

Refinement procedure

Resolution	40.000 - 2.600
R-factor	0.225 *
Rwork	0.225
R-free	0.29200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.240
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.620
High resolution limit [Å]	2.600	2.600
Rmerge	0.054 *	0.174 *
Number of reflections	41570 *
<I/σ(I)>		3.7
Completeness [%]	98.2 *	76.2
Redundancy	3.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	20 *	ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	HEPES	120 (mM)	pH7.5
3	1	reservoir	ammonium sulfate	2.4 (M)
4	1	reservoir	PEG400	2.4 (%(v/v))