1IRX
Crystal structure of class I lysyl-tRNA synthetase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-01-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9792, 0.9795, 0.9824, 0.9724 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.945, 74.767, 156.945 |
Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.600 |
R-factor | 0.225 * |
Rwork | 0.225 |
R-free | 0.29200 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.240 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.620 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.054 * | 0.174 * |
Number of reflections | 41570 * | |
<I/σ(I)> | 3.7 | |
Completeness [%] | 98.2 * | 76.2 |
Redundancy | 3.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 20 * | ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | HEPES | 120 (mM) | pH7.5 |
3 | 1 | reservoir | ammonium sulfate | 2.4 (M) | |
4 | 1 | reservoir | PEG400 | 2.4 (%(v/v)) |