1IRQ
Crystal structure of omega transcriptional repressor at 1.5A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9073 |
| Spacegroup name | P 61 |
| Unit cell lengths | 46.100, 46.100, 88.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.600 - 1.500 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.23200 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 18.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | |
| High resolution limit [Å] | 1.500 | 1.500 * |
| Rmerge | 0.026 * | 0.384 * |
| Total number of observations | 69483 * | |
| Number of reflections | 16703 | |
| Completeness [%] | 97.6 | 95.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 18 * | PEG 3350, 100mM Sodium Acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | glycerol | 5 (%(v/v)) | |
| 5 | 1 | reservoir | PEG3350 | 30 (%(w/v)) | |
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
