1IRO
RUBREDOXIN (OXIDIZED, FE(III)) AT 1.1 ANGSTROMS RESOLUTION
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1993-11 |
Detector | MARRESEARCH |
Wavelength(s) | 0.92 |
Spacegroup name | H 3 |
Unit cell lengths | 64.040, 64.040, 32.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.100 |
R-factor | 0.0903 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXL-93 |
Refinement software | SHELXL-93 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | |
High resolution limit [Å] | 1.100 * | |
Rmerge | 0.047 | 0.262 * |
Number of reflections | 20052 * | |
Completeness [%] | 94.0 * | |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 4 | 23 * | pH 4.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.8-1.6 (mM) | |
2 | 1 | drop | ammonium sulfate | 0.82 (M) | 20%saturated |
3 | 1 | drop | citrate | 30 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 2.46 (M) | 60%saturated |
5 | 1 | reservoir | citrate | 100 (mM) |