1IRK

CRYSTAL STRUCTURE OF THE TYROSINE KINASE DOMAIN OF THE HUMAN INSULIN RECEPTOR

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	54.030, 72.990, 89.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.100
R-factor	0.196
Rwork	0.196
R-free	0.23200
RMSD bond length	0.011
RMSD bond angle	1.580
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.100 *
Rmerge	0.027 *
Number of reflections	37359
Completeness [%]	93.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		21 *	using macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG6000	20 (%)
3	1	reservoir	malate-imidazole	0.2 (M)