1IR8
IM mutant of lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 296 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.390, 79.390, 37.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.630 |
R-factor | 0.166 * |
Rwork | 0.166 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.470 |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 1.630 |
Rmerge | 0.071 * |
Total number of observations | 44733 * |
Number of reflections | 13603 |
Completeness [%] | 94.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.7 | 296 | NaCl, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 296K |