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1IR8

IM mutant of lysozyme

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU300
Temperature [K]296
Detector technologyIMAGE PLATE
DetectorRIGAKU RAXIS IIC
Wavelength(s)1.5418
Spacegroup nameP 43 21 2
Unit cell lengths79.390, 79.390, 37.930
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution6.000 - 1.630
R-factor0.166

*

Rwork0.166
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.009
RMSD bond angle1.470
Phasing softwareX-PLOR
Refinement softwareX-PLOR (3.1)
Data quality characteristics
 Overall
Low resolution limit [Å]15.000
High resolution limit [Å]1.630
Rmerge0.071

*

Total number of observations44733

*

Number of reflections13603
Completeness [%]94.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

4.7296NaCl, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 296K

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PDB entries from 2024-11-13

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