1IR6
Crystal structure of exonuclease RecJ bound to manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 141.600, 141.600, 141.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 2.900 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.25900 |
| Structure solution method | single-wavelength anomalous dispersion |
| RMSD bond length | 0.008 |
| RMSD bond angle | 21.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 * | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.049 * | 0.279 * |
| Total number of observations | 584147 * | |
| Number of reflections | 21198 | |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20 * | ammonium sulfate, sodium/potassium tartrate, sodium citrate, glycerol, manganese chloride, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
| 2 | 1 | reservoir | sodium potassium tartrate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium citrate | 50 (mM) | |
| 4 | 1 | reservoir | glycerol | 10 (%) | pH5.8 |
| 5 | 1 | drop | protein | 10 (mg/ml) | |
| 6 | 1 | drop | Tris-HCl | 20 (mM) | |
| 7 | 1 | drop | 100 (mM) | ||
| 8 | 1 | drop | EDTA | 0.1 (mM) | |
| 9 | 1 | drop | glycerol | 10 (%) | pH7.5 |
