1IR1
Crystal Structure of Spinach Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (Rubisco) Complexed with CO2, Mg2+ and 2-Carboxyarabinitol-1,5-Bisphosphate
Replaces: 1BURExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Detector | WEISSENBERG |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 157.800, 157.800, 200.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| Rwork | 0.155 |
| R-free | 0.17900 |
| Structure solution method | RE-REFINEMENT of 1BUR |
| Starting model (for MR) | PDB ID 1BUR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.360 |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 175568 | |
| Completeness [%] | 76.9 | 47.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 20 * | PEG4000, MgCl2, NaHCO3, DTT, 2-carboxyarabinitol-1,5-bisphosphate, Bicine, pH 7.9, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES-KOH | 80 (mM) | pH8.0 |
| 10 | 1 | reservoir | PEG4000 | 6-7 (%) | |
| 11 | 1 | reservoir | glycerol | 30 (%(v/v)) | |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | drop | 40 (mM) | ||
| 6 | 1 | drop | protein | 25 (mg/ml) | |
| 7 | 1 | reservoir | HEPES-KOH | 80 (mM) | pH8.0 |
| 8 | 1 | reservoir | EDTA | 1 (mM) | |
| 9 | 1 | reservoir | 20 (mM) |
