1IQV
Crystal Structure Analysis of the archaebacterial ribosomal protein S7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-14 |
| Detector | OXFORD PX210 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 58.900, 58.900, 118.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.196 * |
| Rwork | 0.196 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hus |
| RMSD bond length | 0.005 |
| RMSD bond angle | 19.560 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.081 | 0.262 |
| Total number of observations | 139697 * | |
| Number of reflections | 14407 | |
| <I/σ(I)> | 7.2 | 2.9 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 9.7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG3000, sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dithiothreitol | 1 (mM) | |
| 2 | 1 | drop | protein | 10 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.0 |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | PEG3000 | 30 (%(w/v)) |
