1IQU
Crystal structure of photolyase-thymine complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2000-03-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.7 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 113.099, 113.099, 142.554 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.200 |
R-factor | 0.212 |
Rwork | 0.211 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1IQR (native structure) |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.336 | |
Number of reflections | 25481 | |
Completeness [%] | 91.2 | 69.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | sodium acetate, Ammonium phosphate, lithium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |