1IQQ
Crystal Structure of Japanese pear S3-RNase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-25 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.650, 52.590, 47.570 |
Unit cell angles | 90.00, 106.45, 90.00 |
Refinement procedure
Resolution | 500.000 * - 1.500 |
R-factor | 0.172 |
Rwork | 0.172 |
R-free | 0.20200 |
Structure solution method | MIR |
RMSD bond length | 0.022 |
RMSD bond angle | 2.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.890 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.057 * | |
Number of reflections | 33441 | |
Completeness [%] | 96.3 | 90.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 * | Matsuura, T., (2001) Acta Crystallogr. Sect., D57, 172. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | drop | MES/NaOH | 50 (mM) | pH7.0 |
3 | 1 | reservoir | MES-NaOH | 100 (mM) | pH7.0 |
4 | 1 | reservoir | sodium acetate | 200 (mM) | |
5 | 1 | reservoir | PEG6000 | 30 (%(w/v)) |