1IQ7

Ovotransferrin, C-Terminal Lobe, Apo Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE M18XHF
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1997-11-14
Detector	SIEMENS HI-STAR
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	103.580, 81.130, 50.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.186
Rwork	0.186
R-free	0.23900
RMSD bond length	0.005
RMSD bond angle	1.230 *
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.250
Rmerge	0.058 *
Total number of observations	66969 *
Number of reflections	14495
Completeness [%]	70.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25.0 (mg/ml)	pH6.0
2	1	drop	acetate buffer	0.05 (M)
3	1	reservoir	ammonium sulfate	55 (%)	pH6.0
4	1	reservoir	acetate buffer	0.05 (M)