1IQ0
THERMUS THERMOPHILUS ARGINYL-TRNA SYNTHETASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.708 |
| Spacegroup name | P 65 |
| Unit cell lengths | 154.747, 154.747, 84.573 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 * - 2.300 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.24200 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.160 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.051 | 0.186 |
| Number of reflections | 49236 | |
| <I/σ(I)> | 35.8 | 7.4 |
| Completeness [%] | 95.8 | 94.3 |
| Redundancy | 7.94 | 6.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Shimada, A., (2001) Acta Crystallogr., D57, 272. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 100 (mM) | pH7.0 |
| 2 | 1 | drop | PEG8000 | 10 (%(v/v)) | |
| 3 | 1 | drop | ethylene glycol | 8 (%(v/v)) | |
| 4 | 1 | drop | polyvinylpyrrolidone15000 | 2.5 (%) | |
| 5 | 1 | reservoir | ethylene glycol | 8 (%(v/v)) |
