1IPP
HOMING ENDONUCLEASE/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-15 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 113.900, 113.900, 88.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.212 * |
| Rwork | 0.212 |
| R-free | 0.31600 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.048 | 0.193 |
| Total number of observations | 83203 * | |
| Number of reflections | 30702 | |
| <I/σ(I)> | 15 | 3.7 |
| Completeness [%] | 90.0 | 41 |
| Redundancy | 2.7 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.4 | 4 * | DNA:protein solution(in 2:1 ratio) was mixed with an equal volume of reservoir solution * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | EDTA | ||
| 2 | 1 | 1 | SPERMINE | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | SODIUM CITRATE | ||
| 5 | 1 | 1 | PEG 4000 | ||
| 6 | 1 | 2 | PEG 4000 | ||
| 7 | 1 | 2 | SODIUM CITRATE | ||
| 8 | 1 | 2 | NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | I-PpoI | 3 (mg/ml) | |
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | EDTA | 2 (mM) | |
| 4 | 1 | reservoir | citrate | 0.1 (M) | |
| 5 | 1 | reservoir | PEG4000 | 21-27 (%) |
