1IPK
CRYSTAL STRUCTURES OF RECOMBINANT AND NATIVE SOYBEAN BETA-CONGLYCININ BETA HOMOTRIMERS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1996-10-28 |
Detector | SIEMENS HI-STAR |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.510, 63.480, 131.430 |
Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
Resolution | 7.000 - 2.800 * |
R-factor | 0.205 * |
Rwork | 0.205 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 * |
RMSD bond angle | 1.271 * |
Data reduction software | SAINT |
Data scaling software | SAINT |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.800 * |
Rmerge | 0.055 * |
Total number of observations | 101661 * |
Number of reflections | 33007 |
Completeness [%] | 91.0 |
Redundancy | 3.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 * | 4 * | PEG10000, MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
10 | 1 | reservoir | EDTA | 1 (mM) | |
11 | 1 | reservoir | p-APMSF | 0.1 (mM) | |
12 | 1 | reservoir | 0.02 (%) | ||
2 | 1 | drop | citrate | 50 (mM) | pH4.8 |
3 | 1 | drop | 0.4 (M) | ||
4 | 1 | drop | EDTA | 1 (mM) | |
5 | 1 | drop | p-APMSF | 0.1 (mM) | |
6 | 1 | drop | 0.02 (%) | ||
7 | 1 | reservoir | PEG6000 | 5 (%) | |
8 | 1 | reservoir | citrate | 50 (mM) | pH4.8 |
9 | 1 | reservoir | 0.4 (M) |