1IPJ
CRYSTAL STRUCTURES OF RECOMBINANT AND NATIVE SOYBEAN BETA-CONGLYCININ BETA HOMOTRIMERS COMPLEXES WITH N-ACETYL-D-GLUCOSAMINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1998-07-04 |
Detector | SIEMENS HI-STAR |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.780, 69.470, 125.330 |
Unit cell angles | 90.00, 97.22, 90.00 |
Refinement procedure
Resolution | 7.000 - 2.700 |
R-factor | 0.202 * |
Rwork | 0.202 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 * |
RMSD bond angle | 1.217 * |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.700 * |
Rmerge | 0.051 * |
Total number of observations | 72174 * |
Number of reflections | 33726 * |
Completeness [%] | 94.4 |
Redundancy | 2.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 * | 4 * | PEG6000, citrate buffer, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
10 | 1 | reservoir | 0.02 (%) | ||
11 | 1 | reservoir | PEG10000 | 7 (%) | |
12 | 1 | reservoir | MES | 100 (mM) | pH5.5 |
2 | 1 | drop | MES | 100 (mM) | pH5.5 |
3 | 1 | drop | 0.4 (M) | ||
4 | 1 | drop | EDTA | 1 (mM) | |
5 | 1 | drop | PMSF | 1 (mM) | |
6 | 1 | drop | 0.02 (%) | ||
7 | 1 | reservoir | 0.4 (M) | ||
8 | 1 | reservoir | EDTA | 1 (mM) | |
9 | 1 | reservoir | PMSF | 1 (mM) |