1IPJ
CRYSTAL STRUCTURES OF RECOMBINANT AND NATIVE SOYBEAN BETA-CONGLYCININ BETA HOMOTRIMERS COMPLEXES WITH N-ACETYL-D-GLUCOSAMINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-07-04 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.780, 69.470, 125.330 |
| Unit cell angles | 90.00, 97.22, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.700 |
| R-factor | 0.202 * |
| Rwork | 0.202 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.217 * |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.700 * |
| Rmerge | 0.051 * |
| Total number of observations | 72174 * |
| Number of reflections | 33726 * |
| Completeness [%] | 94.4 |
| Redundancy | 2.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 * | 4 * | PEG6000, citrate buffer, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | reservoir | 0.02 (%) | ||
| 11 | 1 | reservoir | PEG10000 | 7 (%) | |
| 12 | 1 | reservoir | MES | 100 (mM) | pH5.5 |
| 2 | 1 | drop | MES | 100 (mM) | pH5.5 |
| 3 | 1 | drop | 0.4 (M) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | PMSF | 1 (mM) | |
| 6 | 1 | drop | 0.02 (%) | ||
| 7 | 1 | reservoir | 0.4 (M) | ||
| 8 | 1 | reservoir | EDTA | 1 (mM) | |
| 9 | 1 | reservoir | PMSF | 1 (mM) |
