1IOZ

Crystal Structure of the C-HA-RAS Protein Prepared by the Cell-Free Synthesis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Detector technology	CCD
Collection date	2000-05-22
Detector	MARRESEARCH
Wavelength(s)	0.97925,0.97882,0.97115,0.98660
Spacegroup name	P 65 2 2
Unit cell lengths	82.645, 82.645, 102.794
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.2385 *
Rwork	0.238
R-free	0.28300
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	0.701 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Rmerge	0.070 *	0.327 *
Total number of observations	232493 *
Number of reflections	14678 *
Completeness [%]	99.8 *	100 *
Redundancy	15.84 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	4 *	calcium acetate, sodium cacodylate, PEG8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	calcium acetate	200 (mM)
2	1	reservoir	sodium cacodylate	100 (mM)
3	1	reservoir	PEG8000	18 (%)