1ION
THE SEPTUM SITE-DETERMINING PROTEIN MIND COMPLEXED WITH MG-ADP FROM PYROCOCCUS HORIKOSHII OT3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979076, 0.979389, 0.89999 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 96.590, 96.590, 96.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.188 * |
| Rwork | 0.228 |
| R-free | 0.22700 * |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 23.450 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.062 | 0.184 |
| Total number of observations | 270283 * | |
| Number of reflections | 13617 | 1953 * |
| <I/σ(I)> | 6.4 | 4.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.8 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 20 * | MPD, SODIUM ACETATE, CALCIUM CHLORIDE, pH 4.60, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.0 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 50 (mM) | |
| 3 | 1 | drop | MPD | 15 (%) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 6 | 1 | reservoir | MPD | 30 (%) | |
| 7 | 1 | reservoir | 20 (mM) |
