1IOK

CRYSTAL STRUCTURE OF CHAPERONIN-60 FROM PARACOCCUS DENITRIFICANS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-11-21
Detector	FUJI
Spacegroup name	P 42 21 2
Unit cell lengths	286.355, 286.355, 153.463
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.200
R-factor	0.2035
Rwork	0.204
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006 *
RMSD bond angle	0.881 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.073	0.464
Total number of observations	582211 *
Number of reflections	103358 *	10279 *
<I/σ(I)>	20.6	3.33
Completeness [%]	98.5	99.3
Redundancy	5.6	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	20 *	PEG 6000, LiCl, Bicine-Na, pH 9.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	9	20 *	PEG 6000, LiCl, Bicine-Na, pH 9.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	60 (mg/ml)
2	1	reservoir	PEG6000	4.0 (%(w/v))
3	1	reservoir		1.0 (M)
4	1	reservoir	Bicine-Na	100 (mM)