1IOB
INTERLEUKIN-1 BETA FROM JOINT X-RAY AND NMR REFINEMENT
Spectrometer
Experimental method: SOLUTION NMR, X-RAY DIFFRACTION (2 Å)
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
Conformers
| Conformers Calculated Total Number | |
| Conformers Submitted Total Number | 1 |
Experimental procedure
| Temperature [K] | 293 |
| Spacegroup name | P 43 |
| Unit cell lengths | 55.140, 55.140, 76.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.25400 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 2.200 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Number of reflections | 10429 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | J. P. Priestle, (1989) Proc. Natl. Acad. Sci. U.S.A., 86, 9667. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 7.6 (mg/ml) | |
| 2 | 1 | 2 | ammonium sulfate | 29 (%(w/v)) |
