1IO1
CRYSTAL STRUCTURE OF F41 FRAGMENT OF FLAGELLIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.750, 36.440, 118.350 |
| Unit cell angles | 90.00, 91.15, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| Rwork | 0.234 |
| R-free | 0.26600 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.523 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.600 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.054 | 0.272 |
| Number of reflections | 121314 | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 98.9 | 97 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 16 * | PEG 6000, NaCl, Glycerol, Iso-Propanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 6 (%) | |
| 3 | 1 | reservoir | glycerol | 12 (%) | |
| 4 | 1 | reservoir | isopropanol | 3-6 (%) | |
| 5 | 1 | reservoir | 50-75 (mM) | ||
| 6 | 1 | reservoir | Tris-HCl | 20 (mM) |
