1INE
HOW THE ANTI-(METAL CHELATE) ANTIBODY CHA255 IS SPECIFIC FOR THE METAL ION OF ITS ANTIGEN: X-RAY STRUCTURES FOR TWO FAB'(SLASH)HAPTEN COMPLEXES WITH DIFFERENT METALS IN THE CHELATE
Experimental procedure
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.900, 85.600, 77.500 |
| Unit cell angles | 90.00, 136.40, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.800 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.600 |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 * |
| Rmerge | 0.070 * |
| Number of reflections | 10900 * |
| Completeness [%] | 81.0 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 10 (%) | |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | drop | HEPES | 50 (mM) | |
| 5 | 1 | reservoir | double the amount of the drop(except for protein) |
