1IMX

1.8 Angstrom crystal structure of IGF-1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-01-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9197, 1.5406, 0.9199, 0.8610
Spacegroup name	C 2 2 21
Unit cell lengths	31.831, 71.055, 65.996
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.000 - 1.820
R-factor	0.245 *
Rwork	0.245
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.006 *
RMSD bond angle	1.054 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNX (2000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.830
High resolution limit [Å]	1.820	1.800
Rmerge	0.038	0.290
Total number of observations	44573 *
Number of reflections	6871
<I/σ(I)>	16.7	3.5
Completeness [%]	98.2 *	99.1
Redundancy	1.8	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.5 *	293	PEG 3350, deoxy big CHAPS, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		0.15 (M)
2	1	drop	sodium acetate	20 (mM)
3	1	drop	protein	10 (mg/ml)
4	1	reservoir	PEG3350	24 (%)
5	1	reservoir	sodium cacodylate	0.1 (M)