1IMH
TonEBP/DNA COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-09-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.568, 95.368, 158.431 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.860 |
R-factor | 0.244 |
Rwork | 0.244 |
R-free | 0.29000 |
Structure solution method | MIRAS |
Starting model (for MR) | 1a02 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.930 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.064 | 0.676 |
Number of reflections | 20432 | |
<I/σ(I)> | 18.2 | 2.77 |
Completeness [%] | 93.9 | 99.9 |
Redundancy | 12 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | PEG 3000, SODIUM CHLORIDE, MAGNESIUM CHLORIDE, BIS-TRIS PROPANE, SODIUM AZIDE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | PEG 3000, SODIUM CHLORIDE, MAGNESIUM CHLORIDE, BIS-TRIS PROPANE, SODIUM AZIDE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | PEG 3000, SODIUM CHLORIDE, MAGNESIUM CHLORIDE, BIS-TRIS PROPANE, SODIUM AZIDE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3000 | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | BIS-TRIS PROPANE | ||
5 | 1 | 1 | SODIUM AZIDE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | bis-Tris-BTP-HCl | 50 (mM) | pH6.6 |
2 | 1 | reservoir | 100 (mM) | ||
3 | 1 | reservoir | PEG3000 | 3-6 (%(w/v)) | |
4 | 1 | reservoir | 10 (mM) | ||
5 | 1 | drop | protein | 0.2-0.4 (mM) | |
6 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
7 | 1 | drop | dithiothreitol | 1 (mM) | |
8 | 1 | drop | 100 (mM) |