1IMD
STRUCTURAL STUDIES OF METAL BINDING BY INOSITOL MONOPHOSPHATASE: EVIDENCE FOR TWO-METAL ION CATALYSIS
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 85.850, 85.850, 153.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 * |
| High resolution limit [Å] | 2.600 * |
| Rmerge | 0.083 * |
| Completeness [%] | 95.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 10 (%) | |
| 2 | 1 | reservoir | glutathione | 5 (mM) | |
| 3 | 1 | reservoir | 5 (mM) | ||
| 4 | 1 | reservoir | 3 (mM) | ||
| 5 | 1 | reservoir | 4-morpholinepropanesulfonic acid | 50 (mM) | |
| 6 | 1 | drop | EGTA | 1 (mM) | |
| 7 | 1 | drop | o-phenanthroline | 2 (mM) | |
| 8 | 1 | drop | Tris | 20 (mM) | |
| 9 | 1 | drop | phosphate | 10-15 (mg/mL) |
