1IMA
STRUCTURAL ANALYSIS OF INOSITOL MONOPHOSPHATASE COMPLEXES WITH SUBSTRATES
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.900, 86.900, 154.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.100 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 * |
| High resolution limit [Å] | 2.300 * |
| Rmerge | 0.079 * |
| Completeness [%] | 93.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 * | Bone, R., (1992) Proc.Nat.Acad.Sci.USA, 89, 10031. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 64-68 (%sat) | ||
| 2 | 1 | reservoir | glutathione | 5 (mM) | |
| 3 | 1 | reservoir | 3 (mM) | ||
| 4 | 1 | reservoir | 2-6 (mM) | can be replaced with 5mM CaSO4 | |
| 5 | 1 | drop | inositol monophosphatase | 10 (mg/ml) | |
| 6 | 1 | drop | Tris-HCl | 20 (mM) | |
| 7 | 1 | drop | EGTA | 1 (mM) | |
| 8 | 1 | drop | o-phenanthroline | 2 (mM) |
