1IM8
Crystal structure of YecO from Haemophilus influenzae (HI0319), a methyltransferase with a bound S-adenosylhomocysteine
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-07-03 |
| Detector | BRUKER |
| Wavelength(s) | 0.9782, 0.9778, 0.9537 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 74.970, 74.970, 319.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.192 * |
| Rwork | 0.186 |
| R-free | 0.25700 * |
| Structure solution method | MAD |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 1.600 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.059 | 0.351 |
| Total number of observations | 174537 * | |
| Number of reflections | 51819 * | |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 93.7 * | 74.6 |
| Redundancy | 3.2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 295 | 20 % saturated ammonium sulfate, 100mM NaHepes with protein solution (10mg/ml in 150mM NaCl, 10mM NaHepes, pH 8.0, 1mM DTT and 0.5mM EDTA) , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | sodium HEPES | 10 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | EDTA | 0.5 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 20 (%sat) | |
| 7 | 1 | reservoir | sodium HEPES | 0.1 (M) |
