1IM4
Crystal Structure of a DinB Homolog (DBH) Lesion Bypass DNA Polymerase Catalytic Fragment from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-16 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.988, 87.542, 76.983 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.910 - 2.300 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.26100 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.041 | 0.331 |
| Number of reflections | 12897 | |
| <I/σ(I)> | 38.4 | 3.8 |
| Completeness [%] | 97.7 | 97.8 |
| Redundancy | 6.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | ammonium sulfate, magnesium chloride, MPD, Tris-HCl, beta-mercaptoethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 60 (mg/ml) | |
| 10 | 1 | reservoir | beta-mercaptoethanol | 5 (mM) | |
| 2 | 1 | drop | HEPES-NaOH | 20 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
| 5 | 1 | drop | EDTA | 0.5 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 8 | 1 | reservoir | 10 (mM) | ||
| 9 | 1 | reservoir | MPD | 2 (%(v/v)) |
