1ILU

X-RAY CRYSTAL STRUCTURE THE TWO SITE-SPECIFIC MUTANTS ILE7SER AND PHE110SER OF AZURIN FROM PSEUDOMONAS AERUGINOSA

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-05-15
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	41.400, 170.100, 99.700
Unit cell angles	90.00, 91.30, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.171
Rwork	0.171
RMSD bond length	0.012
RMSD bond angle	24.800 *
Data reduction software	MOSFLM
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.300	2.280 *
Rmerge	0.106
Total number of observations	81888 *
Number of reflections	54140
Completeness [%]	86.3	74.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	sodium acetate	0.1 (M)
3	1	drop	ammonium sulfate	2.5 (M)
4	1	drop	lithium nitrate	0.6 (M)
5	1	reservoir	ammonium sulfate	3.5 (M)
6	1	reservoir	lithium nitrate	1.0 (M)