1IL4
STRUCTURE OF RICIN A CHAIN BOUND WITH INHIBITOR 9-DEAZAGUANINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-18 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.700, 68.410, 50.160 |
| Unit cell angles | 90.00, 112.80, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.222 * |
| Rwork | 0.222 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rtc |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.196 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.084 | 0.288 |
| Total number of observations | 16235 * | |
| Number of reflections | 8286 | |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 94.0 | 87.2 * |
| Redundancy | 1.96 | 1.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.9 | 4.0 * | Mlsna, D., (1993) Protein Sci., 2, 429. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.2-2.4 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 75 (mM) | pH8.9 |
| 3 | 1 | reservoir | beta-mercaptoethanol | 10 (mM) | |
| 4 | 1 | reservoir | EDTA | 1 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 4.1 (%) |
