1IL2
Crystal Structure of the E. coli Aspartyl-tRNA Synthetase:Yeast tRNAasp:aspartyl-Adenylate Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.800, 222.800, 80.800 |
| Unit cell angles | 90.00, 111.80, 90.00 |
Refinement procedure
| Resolution | 11.500 - 2.600 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | T. thermophilus AspRS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Phasing software | CCP4 ((ALMN) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.500 | |
| High resolution limit [Å] | 2.600 | |
| Rmerge | 0.037 * | 0.216 * |
| Number of reflections | 72094 * | |
| Completeness [%] | 92.0 * | 80 * |
| Redundancy | 2.3 | 2.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | Boeglin, M., (1996) Acta Crystallogr, D52, 211. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulfate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | AspRS | 2 (mg/ml) | |
| 10 | 1 | reservoir | 2-propanol | 1-3 (%(v/v)) | |
| 2 | 1 | drop | tRNA | 1 (mg/ml) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | ammonium sulfate | 1.5 (M) | |
| 5 | 1 | drop | Bis-Tris-propane | 75 (mM) | |
| 6 | 1 | drop | AMP-PCP | 0.5 (mM) | |
| 7 | 1 | drop | L-aspartic acid | 1 (mM) | |
| 8 | 1 | reservoir | ammonium sulfate | 1.9 (M) | |
| 9 | 1 | reservoir | Bis-Tris-propane | 100 (mM) |
