1IL2
Crystal Structure of the E. coli Aspartyl-tRNA Synthetase:Yeast tRNAasp:aspartyl-Adenylate Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.800, 222.800, 80.800 |
Unit cell angles | 90.00, 111.80, 90.00 |
Refinement procedure
Resolution | 11.500 - 2.600 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | T. thermophilus AspRS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Phasing software | CCP4 ((ALMN) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 11.500 | |
High resolution limit [Å] | 2.600 | |
Rmerge | 0.037 * | 0.216 * |
Number of reflections | 72094 * | |
Completeness [%] | 92.0 * | 80 * |
Redundancy | 2.3 | 2.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | Boeglin, M., (1996) Acta Crystallogr, D52, 211. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ammonium sulfate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | AspRS | 2 (mg/ml) | |
10 | 1 | reservoir | 2-propanol | 1-3 (%(v/v)) | |
2 | 1 | drop | tRNA | 1 (mg/ml) | |
3 | 1 | drop | 1 (mM) | ||
4 | 1 | drop | ammonium sulfate | 1.5 (M) | |
5 | 1 | drop | Bis-Tris-propane | 75 (mM) | |
6 | 1 | drop | AMP-PCP | 0.5 (mM) | |
7 | 1 | drop | L-aspartic acid | 1 (mM) | |
8 | 1 | reservoir | ammonium sulfate | 1.9 (M) | |
9 | 1 | reservoir | Bis-Tris-propane | 100 (mM) |