1IL1

Crystal structure of G3-519, an anti-HIV monoclonal antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	296
Detector technology	IMAGE PLATE
Collection date	1997-04-22
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	37.200, 84.300, 134.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.740 - 2.200
R-factor	0.187
Rwork	0.188
R-free	0.24700 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.740	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.100	0.505
Number of reflections	18605
<I/σ(I)>	12.4
Completeness [%]	90.2	88.1
Redundancy	3	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	296	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2 (M)
2	1	reservoir		200 (mM)
3	1	reservoir	sodium citrate	100 (mM)