1IL1
Crystal structure of G3-519, an anti-HIV monoclonal antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 296 |
Detector technology | IMAGE PLATE |
Collection date | 1997-04-22 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.200, 84.300, 134.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.740 - 2.200 |
R-factor | 0.187 |
Rwork | 0.188 |
R-free | 0.24700 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.740 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.100 | 0.505 |
Number of reflections | 18605 | |
<I/σ(I)> | 12.4 | |
Completeness [%] | 90.2 | 88.1 |
Redundancy | 3 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 296 | used microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2 (M) | |
2 | 1 | reservoir | 200 (mM) | ||
3 | 1 | reservoir | sodium citrate | 100 (mM) |