1IKO
CRYSTAL STRUCTURE OF THE MURINE EPHRIN-B2 ECTODOMAIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-02 |
Detector | SBC-2 |
Wavelength(s) | 1.033 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 59.283, 59.283, 168.868 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.410 - 1.920 |
R-factor | 0.215 * |
Rwork | 0.216 |
R-free | 0.24800 * |
Structure solution method | SIRAS |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.600 * |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.500 * | 1.990 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.042 * | 0.219 * |
Total number of observations | 315392 * | |
Number of reflections | 14283 * | |
<I/σ(I)> | 48 | 18 |
Completeness [%] | 99.5 | 99.8 |
Redundancy | 22 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | magnesium chloride, PEG 4000, trishydroxyethylmethane, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30-98 (mg/ml) | |
2 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
3 | 1 | reservoir | 200 (mM) | ||
4 | 1 | reservoir | PEG4000 | 20 (%) |