1IKE
Crystal Structure of Nitrophorin 4 from Rhodnius Prolixus Complexed with Histamine at 1.5 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 140 |
| Detector technology | DIFFRACTOMETER |
| Detector | ENRAF-NONIUS FAST |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.180, 42.520, 52.860 |
| Unit cell angles | 90.00, 94.35, 90.00 |
Refinement procedure
| Resolution | 8.900 - 1.500 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.24000 |
| Starting model (for MR) | 1d2u |
| Data reduction software | MADNESS |
| Data scaling software | SCALA |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.900 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.070 | 0.220 |
| Number of reflections | 20817 | |
| <I/σ(I)> | 11.6 | 2.9 |
| Completeness [%] | 93.5 | 76 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | PEG4000, Sodium Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
