1IK9
CRYSTAL STRUCTURE OF A XRCC4-DNA LIGASE IV COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-02-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.062, 79.628, 242.142 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.260 - 2.300 |
R-factor | 0.228 * |
Rwork | 0.228 |
R-free | 0.26600 |
Structure solution method | SAS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.260 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.051 | 0.229 |
Total number of observations | 72477 * | |
Number of reflections | 34224 | |
<I/σ(I)> | 17.9 | 2.3 |
Completeness [%] | 85.5 | 44.4 |
Redundancy | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | used seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.050 (mM) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | PEG6000 | 1.5 (%(w/v)) | |
5 | 1 | drop | MES | 0.05 (M) | pH6.0 |
7 | 1 | drop | xylitol precipitant solution | 1.5 (%(w/v)) | |
8 | 1 | reservoir | PEG6000 | 6 (%(w/v)) | |
9 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |