1IK7
Crystal Structure of the Uncomplexed Pelle Death Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9470 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 70.106, 95.542, 103.196 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 * - 2.300 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.25900 * |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 0.840 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.063 | 0.256 |
Number of reflections | 15711 | |
<I/σ(I)> | 19.6 | 3 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 5.6 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 * | 4 * | 45% 2-methyl-2,4-pentanediol, Tris, 0.4 M sodium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
1 | Vapor diffusion * | 7.5 * | 4 * | 45% 2-methyl-2,4-pentanediol, Tris, 0.4 M sodium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
1 | Vapor diffusion * | 7.5 * | 4 * | 45% 2-methyl-2,4-pentanediol, Tris, 0.4 M sodium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-Cl | 20 (mM) | pH7.5 |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | EDTA | 1 (mM) | |
6 | 1 | reservoir | MPD | 40-46 (%) | |
7 | 1 | reservoir | 0.4-0.6 (M) | ||
8 | 1 | reservoir | Tris-Cl | 0.1 (M) | pH8.0 |