1IK5
Crystal Structure of a 14mer RNA Containing Double UU Bulges in Two Crystal Forms: A Novel U*(AU) Intramolecular Base Triple
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-02 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.040, 29.530, 52.440 |
| Unit cell angles | 90.00, 120.83, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.209 * |
| Rwork | 0.209 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.440 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.060 * |
| Number of reflections | 7100 * |
| Completeness [%] | 91.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | cacodylate, magnesium chloride, MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | cacodylate | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 1 (mM) | double strand |
| 2 | 1 | drop | spermine tetrahydrochloride | 10 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 4 | 1 | drop | 10 (%(v/v)) | ||
| 5 | 1 | drop | 20 (mM) | ||
| 6 | 1 | reservoir | MPD | 40 (%) |
