1IJS
CPV (STRAIN D) mutant A300D, complex (VIRAL COAT/DNA), VP2, PH=7.5, T=4 DEGREES C
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-09-15 |
| Detector | FUJI |
| Spacegroup name | P 1 |
| Unit cell lengths | 267.600, 268.500, 274.300 |
| Unit cell angles | 61.90, 62.60, 60.20 |
Refinement procedure
| Resolution | ? - 3.250 |
| R-factor | 0.159 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CANINE PARVOVIRUS (MONOCLINIC P21) |
| Data reduction software | PURDUE (SOFTWARE) |
| Data scaling software | PURDUE (DATA PROCESSING PACKAGE) |
| Phasing software | PURDUE (AVERAGING PROGRAM) |
| Refinement software | NONE |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 3.250 * |
| Rmerge | 0.090 |
| Redundancy | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | Tsao, J., (1992) Acta Crystallogr.,Sect.B, 48, 75. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 8000 | ||
| 3 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
| 4 | 1 | 1 | CACL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 0.75 (%) | |
| 2 | 1 | reservoir | 6 (mM) | ||
| 3 | 1 | reservoir | Tris-HCl | 10 (mM) | pH7.5 |
