1IJK
The von Willebrand Factor mutant (I546V) A1 domain-botrocetin Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 111 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.989 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.664, 66.672, 69.402 |
| Unit cell angles | 90.00, 105.41, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.600 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.320 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.046 | 0.207 |
| Total number of observations | 49341 * | |
| Number of reflections | 18178 | 594 * |
| <I/σ(I)> | 23.9 | |
| Completeness [%] | 92.9 | 62.5 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 4000, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 3 | 1 | reservoir | PEG4000 | 16 (%) |
