1IJG

Structure of the Bacteriophage phi29 Head-Tail Connector Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-08-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	176.753, 171.568, 184.707
Unit cell angles	90.00, 112.21, 90.00

Refinement procedure

Resolution	500.000 - 2.900
R-factor	0.225
Rwork	0.224
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fou
RMSD bond length	0.008
RMSD bond angle	1.216
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.920
High resolution limit [Å]	2.900	2.900
Rmerge	0.088	0.333
Number of reflections	110311
<I/σ(I)>	28.9
Completeness [%]	97.8	99 *
Redundancy	3.54 *	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8	298	MPD, calcium cloride, tris buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	MPD	36-40 (%)
2	1	1		0.05 (M)
3	1	1	Tris-HCl	0.1 (M)