1IJG
Structure of the Bacteriophage phi29 Head-Tail Connector Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.753, 171.568, 184.707 |
| Unit cell angles | 90.00, 112.21, 90.00 |
Refinement procedure
| Resolution | 500.000 - 2.900 |
| R-factor | 0.225 |
| Rwork | 0.224 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fou |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.216 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.920 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.088 | 0.333 |
| Number of reflections | 110311 | |
| <I/σ(I)> | 28.9 | |
| Completeness [%] | 97.8 | 99 * |
| Redundancy | 3.54 * | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 | 298 | MPD, calcium cloride, tris buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | MPD | 36-40 (%) | |
| 2 | 1 | 1 | 0.05 (M) | ||
| 3 | 1 | 1 | Tris-HCl | 0.1 (M) |
