1IJD

Crystallographic Structure of the LH3 Complex from Rhodopseudomonas acidophila strain 7050

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-08-29
Detector	MARRESEARCH
Wavelength(s)	0.87
Spacegroup name	H 3 2
Unit cell lengths	117.260, 117.260, 295.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.000 - 3.000
R-factor	0.243 *
Rwork	0.243
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1KZU (LH2 complex from Rps. acidophila strain 10050)
RMSD bond length	0.013 *
RMSD bond angle	0.021 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	AMoRE
Refinement software	RESTRAIN

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.000	2.870
High resolution limit [Å]	3.000 *	2.800
Rmerge	0.085 *	0.370 *
Number of reflections	18970
<I/σ(I)>	4.6	1.3
Completeness [%]	97.0 *	96.4 *
Redundancy	3.7	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.2	291	McLuskey, K., (1999) Acta Crystallogr., Sect.D, 55, 885. *
1	VAPOR DIFFUSION, SITTING DROP	9.2	291	McLuskey, K., (1999) Acta Crystallogr., Sect.D, 55, 885. *
1	VAPOR DIFFUSION, SITTING DROP	9.2	291	McLuskey, K., (1999) Acta Crystallogr., Sect.D, 55, 885. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	potassium phosphate	4.0 (M)	pH9.2
2	1	drop	benzamidine hydrochloride	2.5 (%(w/v))
3	1	reservoir	ammonium sulfate	2.3 (M)	pH9.7