1IJ8

CRYSTAL STRUCTURE OF LITE AVIDIN-BNI COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2000-06-18
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 2
Unit cell lengths	72.269, 80.362, 43.402
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.204 *
Rwork	0.204
R-free	0.23400
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2avi
RMSD bond length	0.006 *
RMSD bond angle	1.350 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.052	0.279
Number of reflections	17510
<I/σ(I)>	30.7
Completeness [%]	98.7	98.1
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3 *	20 *	12% PEG2000, 0.1M imidazole malate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.2 (mg/ml)
2	1	reservoir	PEG2000	15 (%)
3	1	reservoir	phosphate	0.1 (M)