1IJ3

GCN4-pVSL Coiled-coil trimer with Serine at the a(16) position

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.2830
Spacegroup name	P 1
Unit cell lengths	25.564, 25.839, 34.373
Unit cell angles	86.47, 84.49, 79.40

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.22
Rwork	0.220
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.059	0.180
Total number of observations	23291 *
Number of reflections	7378
<I/σ(I)>	12.1	3.5
Completeness [%]	92.6	89 *
Redundancy	1.9	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sparse matrix method *	6.5	298	0.1 M MES, 50 mM cesium chloride, 10% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	MES	0.1 (M)
2	1	1		50 (mM)
3	1	1	PEG20000	10 (%)