1IJ3
GCN4-pVSL Coiled-coil trimer with Serine at the a(16) position
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.2830 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.564, 25.839, 34.373 |
| Unit cell angles | 86.47, 84.49, 79.40 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 | 0.180 |
| Total number of observations | 23291 * | |
| Number of reflections | 7378 | |
| <I/σ(I)> | 12.1 | 3.5 |
| Completeness [%] | 92.6 | 89 * |
| Redundancy | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sparse matrix method * | 6.5 | 298 | 0.1 M MES, 50 mM cesium chloride, 10% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | MES | 0.1 (M) | |
| 2 | 1 | 1 | 50 (mM) | ||
| 3 | 1 | 1 | PEG20000 | 10 (%) |
