1IIH
STRUCTURE OF TRYPANOSOMA BRUCEI BRUCEI TRIOSEPHOSPHATE ISOMERASE COMPLEXED WITH 3-PHOSPHOGLYCERATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Detector technology | DIFFRACTOMETER |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 112.360, 97.590, 46.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.14 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.500 |
| Data reduction software | MADNESS |
| Data scaling software | BIOMOL |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.200 | 2.200 * |
| Rmerge | 0.051 | |
| Completeness [%] | 72.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | triosephosphate isomerase | 4.5 (mg/ml) | 0.9 M ammonium sulphate |
| 2 | 1 | drop | ammonium sulfate | 60 (%) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | 3-(N-morpholino)-propane-sulphonic acid | 0.2 (M) | |
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | drop | sodium azide | 1 (mM) | |
| 7 | 1 | reservoir | ammonium sulphate | 60 (%) |
