1IIG
STRUCTURE OF TRYPANOSOMA BRUCEI BRUCEI TRIOSEPHOSPHATE ISOMERASE COMPLEXED WITH 3-PHOSPHONOPROPIONATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-21 |
Detector technology | DIFFRACTOMETER |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 112.360, 97.590, 46.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.125 |
RMSD bond length | 0.014 |
RMSD bond angle | 2.500 |
Data reduction software | MADNESS |
Data scaling software | BIOMOL |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.600 | 2.600 * |
Rmerge | 0.023 | |
Completeness [%] | 75.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | triosephosphate isomerase | 4.5 (mg/ml) | 0.9 M ammonium sulphate |
2 | 1 | drop | ammonium sulfate | 60 (%) | |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | 3-(N-morpholino)-propane-sulphonic acid | 0.2 (M) | |
5 | 1 | drop | dithiothreitol | 1 (mM) | |
6 | 1 | drop | sodium azide | 1 (mM) | |
7 | 1 | reservoir | ammonium sulphate | 60 (%) |