1IIB
CRYSTAL STRUCTURE OF IIBCELLOBIOSE FROM ESCHERICHIA COLI
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.791, 31.782, 60.253 |
| Unit cell angles | 90.00, 101.70, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.24100 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 21.400 * |
| Data reduction software | XDS |
| Data scaling software | BIOMOL |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.061 | 0.122 |
| Number of reflections | 16165 | |
| Completeness [%] | 82.8 | 74.1 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 4 * | macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.25 (mg/ml) | |
| 10 | 1 | reservoir | 1 (mM) | ||
| 2 | 1 | drop | PEG4000 | 16-16.5 (%(w/v)) | |
| 3 | 1 | drop | BES/NaOH | 0.05 (M) | |
| 4 | 1 | drop | 0.5 (mM) | ||
| 5 | 1 | drop | 2-propanol | 5 (%(v/v)) | |
| 6 | 1 | drop | benzamidine/HCl | 0.5 (%(w/v)) | |
| 7 | 1 | reservoir | PEG4000 | 18-19 (%) | |
| 8 | 1 | reservoir | 2-propanol | 5 (%(v/v)) | |
| 9 | 1 | reservoir | BES/NaOH | 0.1 (M) |
