1II7
Crystal structure of P. furiosus Mre11 with manganese and dAMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-07-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98400 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.290, 88.740, 145.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.26400 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.046 | 0.326 |
Total number of observations | 431781 * | |
Number of reflections | 48885 * | |
<I/σ(I)> | 12 | |
Completeness [%] | 98.1 | 86.1 |
Redundancy | 8.8 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 4 * | 100 mM Ches, 400 mM ammonium sulfate, 35% OEG 6K, 1 mM manganese, 10 mM dAMP, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | drop | phosphate | 20 (mM) | |
3 | 1 | drop | 200 (mM) | ||
4 | 1 | drop | EDTA | 0.1 (mM) | |
5 | 1 | drop | glycerol | 5 (%) | |
6 | 1 | reservoir | ches | 100 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 400 (mM) | |
8 | 1 | reservoir | PEG6000 | 35 (%) |