1II5
CRYSTAL STRUCTURE OF THE GLUR0 LIGAND BINDING CORE COMPLEX WITH L-GLUTAMATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-06-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.866, 49.706, 55.572 |
| Unit cell angles | 90.00, 116.84, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.600 |
| R-factor | 0.244 |
| Rwork | 0.216 |
| R-free | 0.26300 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.055 | 0.120 |
| Total number of observations | 179108 * | |
| Number of reflections | 31427 | |
| <I/σ(I)> | 22.8 | 8.4 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 4 * | 38% MPD, 100 mM sodium acetate, 10 mM L-glutamic acid, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 34-38 (%) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 4 | 1 | drop | L-glutamate | 10 (mM) |
